![]() When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates. SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. ![]() Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)Ĭompare and learn more about our western blot chemiluminescent substrates ›.Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL) There are a lot of reasons why researchers all over the world choose enhanced chemiluminescence (ECL) detection for a wide range of Western blot applications.Compatibility: nitrocellulose and PVDF membranes.Pierce ECL Western Blotting Substrate enables the detection of picogram amounts of antigen. solution at 0 ☌, followed by heating at 50 ☌. Thermo Scientific Pierce ECL Western Blotting Substrate is an, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. Stability: 8-hr working solution stability, 1-year kit stability at 4☌ Western blotting is the most extensively used technique for the identification and characterisation of proteins and their expression levels. 5) Detection of signal 1) Prepare working luminol solution (ECL solution for Western blotting) according to the manufacturer's instructions 2) Drain off.Sensitivity: low-femtogram (mid-zeptomole).SuperSignal West Femto Maximum Sensitivity Substrate characteristics include: Stripping, blocking, and then re-probing primary and. From what I read and heard, there are three ways to re-developing signal in western blot: 1. Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. No need to change primary and secondary antibody. Western blot analysis of diluted recombinant human Akt-1 protein using Akt (pan) (C67E7) Rabbit mAb 4691. ECL Western Blot Substrates ECL substrates for high-sensitivity western blot detection Thermo Scientific SuperSignal ECL substrates are HRP substrates that offer excellent performance in western blotting applications, with longer light emission and stronger signal intensity than other luminol-based or acridan-based detection systems.
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